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Objective: Clinical and basic research increasingly suggests a correlation between migraine and irritable bowel syndrome [IBS]. In this study, we aimed to explore the clinical features and risk factors for IBS in migraine patients
Methods: This was a retrospective, cross-sectional study. A total of 1,112 consecutive patients from the internal medicine and emergency departments of three hospitals from June 2014 through 2016. A comprehensive interviewer-administered questionnaire was designed based on the International Classification of Headache Disorders, 3rd edition [beta version]
Results: The response rate was 94.6%. Among 1,052 participants, 287 suffered from migraine [27.3%] and 312 suffered from IBS [29.7%]. A total of 79 patients suffered from both migraine and IBS [comorbidity rate: 7.5%]. The migraine cohort exhibited a higher frequency of IBS than did the comparison cohort at baseline [P<0.05]. Migraine patients with higher headache frequency, longer length of headache history, and anxiety disorders were more likely to also suffer from IBS [P=0.015]. There were no significant differences between the two groups in age, sex, family history, duration of headache attack, migraine aura, headache intensity, or depression disorders [P>0.05]. Multiple regression analysis indicated length of headache history and headache frequency were associated with IBS
Conclusion: Migraine patients with a long headache history, recurrent episodic headache attacks, and anxiety were more likely to have IBS
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Objective:To optimize the extraction process of total flavonoids from Chloranthus japonicus sieb by Box-Behnken re-sponse surface methodology. Methods:The independent variables were the ethanol concentration ( X1 ) , liquid-solid ratio ( X2 ) and extraction time ( X3 ) , the dependent variable was the extraction amount of total flavonoids ( Y) , and the extraction process was opti-mized by Box-Behnken response surface methodology. Results: The results showed that the optimal extraction conditions for total fla-vonoids from Chloranthus japonicus sieb were as follows: the ethanol concentration was 54. 8%, the liquid-solid ratio was 13. 6, and the extraction time was 2. 0 h. The verified extraction process parameters of three batches showed that the relative error between the ex-perimental values and those predicted from the regression model was -2. 34%. Conclusion:The optimal extraction process parameters are simple and more convenient with higher precision for the extraction of total flavonoids from Chloranthus japonicus sieb.
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Objective To assess the characteristic of normal tension glaucoma (NTG) patients with mild cogni?tive impairment (MCI). Methods This study included twenty-six cases of normal tension glaucoma patients who were diagnosed at ophthalmology department of Peking University Third Hospital. All the participants were examined by us?ing the scales of Montreal Cognitive Assessment (MoCA), mini-mental state examination(MMSE), clinical dementia rat?ing (CDR), activities of daily living (ADL), Hamilton anxiety scale (HAMA), Hamilton depression scale (HAMD), Pitts?burgh Sleep Quality Index (PSQI) and polysomnography (PSG). The patients were then divided into Group with MCI (16 cases) and Group without MCI (19 cases). Results There was no difference between the two groups in gender, age, edu?cation, depression, anxiety and body mass index (BMI) (P>0.05), but significant difference in MoCA scores (P0.05). The in?cidence rate of sleep structure disorder of PSG in all the NTG patients was 85.7%(30/35 patients), 87.5%(14/16 pa?tients) in Group with MCI, and 84.2%(16/19 patients) in Group without MCI. Sleep time of NREM-N3 was significant?ly shorter in Group with MCI than in Group without MCI (P0.05). Conclusion NTG patients with mild cogni?tive impairment are more prone to sleep disorders, especially sleep structure disturbance and short NREM-N3 time may affect cognitive function.
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Objective To study the expressive levels of galectin-3 (gal-3) and sambucus nigra agglutinin(SNA) and to detect their clinicopathological significances in benign and malignant lesions of the gallbladder. Methods EnVisonTM Immunohistochemistry for assaying gal-3 expressive levels and ABC cytochemistry for determining SNA expressive levels were used in conventional paraffin-embedded sections from the specimens of adenocarcinoma (n = 108) , peritumoral tissues (n=46) . Adenomatous polyp (n=15), and chronic cholecystitis (n = 35). Results The positive rates of gal-3 and SNA were significantly higher in adenocarcinoma (62. 0%, 66. 7%) than in peritumoral tissues (39. 1%,45.6%), adenomatous polyp (26.7%, 33.3%) and chronic cholecystitis (11.4%, 11.4%)(P<0. 05 or P<0. 01). The positive gal-3 and SNA in benign cases showed atypical hyperplasia of the epthelium. The positive rates of gal-3 and SNA expression were significantly lower in well-differentiated adenocarcioma, mass with a maximal diameter of <2 cm, absence of lymph node metastasis,and absence of invasion to adjacent tissues than in poor-differentiated adenocarcinoma, mass with a maximal diameter of ≥2 cm, lymph node metastasis and invasion to adjacent tissues. (P<0. 05 or P<0. 01). There was a significant correlation between the expressive levels of gal-3 and SNA in gallbladder adenocarcinoma (x2=9. 51, P<0. 01). Conclusions The expressive levels of gal-3 and SNA lectins had important effects on carcinogenesis, progression and biologic behaviors of gallbladder cancer. Patients with positive gal-3 and /or SNA expressions had poor prognosis.
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AIM: To investigate the molecular mechanism of Epstein-Barr virus encoded latent membrane protein 1 regulated cellular proliferation in nasopharyngeal epithelial cells. METHODS: The nasopharyngeal epithelial cells NP69 were infected with RV-pLNSX (the empty vector) and RV-LMP1 retroviruses, respectively. Therefore, the NP69-pLNSX and NP69-LMP1 cell lines were established. Sequentially, cellular proliferation of NP69-pLNSX and NP69-LMP1 cells was compared to draw the cellular growth curve. The experiments of plate clone formation and forming of soft agar colony were conducted. Meanwhile, the differential expression of proteins were identified between NP69-pLNSX and NP69-LMP1 cell lines by proteomic methods, and the expression levels of partial identified proteins were verified. RESULTS: (1) LMP1 was able to accelerate cellular proliferation of nasopharyngeal epithelial cell NP69 (n=3, P<0.05). (2) Twenty two proteins (9 up-and 13 down-regulated) of LMP1 mediated regulation were identified from infected NP69 cell lines, and the differential expression of partial identified proteins was confirmed by Western blotting and fluorescent real-time quantitative RT-PCR. CONCLUSION: LMP1 probably mediates the regulation of vimentin protein and keratin 19 protein expression to promote cellular proliferation in NP69 cells.
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Objective To study the expressions of CA19-9 and CA125 and their clinicopathologic significances in gallbladder adenocarcinoma, pericancerous tissues and chronic cholecystitis. Methods EnVisionTM immunohistochemistry was used for assaying the expressive levels of CA19-9 and CA125 in the routinely paraffin-embedded sections of specimens from gallbladder adenocarcinoma (n=108), pericancerous tissues (n=46), and chronic cholecystitis (n=35). Results The positive rates of CA19-9 and CA125 were significantly higher in gallbladder adenocarcinoma (49.1%, 51.9%) than those in pericancerous tissues (26.1%, 15.2%) and chronic cholecystitis (14.3%, 5.7%), respectively (P
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Objective To explore the diagnosis,treatment and prognosis of male breast cancer.Methods The clinical data of 17 male patients with breast cancer were analyzed retrospectively.Results The average age of these 17 patients was 59.6 years.In these 17 male cases,the breast cancer in 3 cases was stageⅠ,in 5 cases stageⅡ,in 7 cases stage Ⅲ,and in 2 cases stage Ⅳ.The major pathological type was typical invasive ductal carcinoma.The breast cancer positive rates of estrogen receptor was 82.4 % and progestogen receptor(PR) was 72.5 %.All of these cases were treated by radical operation and postoperative adjuvant radiotherapy,endocrine therapy and(or) chemotherapy.One patient was lost to follow-up,2 died of non-tumor disease 8 months and 3 years after operation,respectively,and the other 14 have survived for 1-12 years after operation.Conclusions Male breast cancer is an uncommon disease,age of onset is more advanced,and the misdiagnostic rate is high.Tamoxifen is the first choice of hormone therapy.Many factors influence the prognosis of male breast cancer,the most important of which are the TNM stage of tumor,and condition of lymph node involvement.
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0.05). Short-term memory and logic memory(LM) scores were positively correlated with entorhinal cortex volumes(r=0.484, 0.529; all P